![]() A prediction from these results is that overexpression of an SR protein, such as ASF/SF2, during virus growth will interfere with virus replication by disturbing the balance of functional and nonfunctional ASF/SF2 in the infected cell. ![]() We have previously shown that SR proteins late during an adenovirus infection become partially inactivated as splicing regulatory proteins. Interestingly, ASF/SF2, which is a prototypical member of the SR family of splicing factors, has the opposite effect on splice site selection, inducing an increase in proximal splice site usage. ![]() The general tendency is that splice site selection changes from proximal to distal late after infection. ![]() Expression of cytoplasmic mRNA from most adenovirus transcription units is subjected to a temporal regulation at the level of alternative pre-mRNA splicing.
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